Identification of synthetic vaccine candidates against SARS CoV infection.
Identifieur interne : 000E84 ( Main/Exploration ); précédent : 000E83; suivant : 000E85Identification of synthetic vaccine candidates against SARS CoV infection.
Auteurs : Shu-Pei Lien [République populaire de Chine] ; Yi-Ping Shih ; Hsin-Wei Chen ; Jy-Ping Tsai ; Chih-Hsiang Leng ; Min-Han Lin ; Li-Hsiu Lin ; Hsin-Yu Liu ; Ai-Hsiang Chou ; Yu-Wen Chang ; Yi-Ming A. Chen ; Pele Chong ; Shih-Jen LiuSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2007.
Descripteurs français
- KwdFr :
- Adjuvant Freund, Animaux, Cochons d'Inde, Conception de médicament, Interféron gamma (métabolisme), Lapins, Lymphocytes T (métabolisme), Lymphocytes auxiliaires Th1, Peptides (), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (métabolisme), Tests de neutralisation, Vaccins synthétiques (), Épitopes ().
- MESH :
- métabolisme : Interféron gamma, Lymphocytes T, Syndrome respiratoire aigu sévère.
- Adjuvant Freund, Animaux, Cochons d'Inde, Conception de médicament, Lapins, Lymphocytes auxiliaires Th1, Peptides, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Tests de neutralisation, Vaccins synthétiques, Épitopes.
English descriptors
- KwdEn :
- Animals, Drug Design, Epitopes (chemistry), Freund's Adjuvant, Guinea Pigs, Interferon-gamma (metabolism), Mice, Mice, Inbred BALB C, Neutralization Tests, Peptides (chemistry), Rabbits, Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (prevention & control), T-Lymphocytes (metabolism), Th1 Cells, Vaccines, Synthetic (chemistry).
- MESH :
- chemical , chemistry : Epitopes, Peptides, Vaccines, Synthetic.
- chemical , metabolism : Interferon-gamma.
- metabolism : Severe Acute Respiratory Syndrome, T-Lymphocytes.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Drug Design, Freund's Adjuvant, Guinea Pigs, Mice, Mice, Inbred BALB C, Neutralization Tests, Rabbits, Th1 Cells.
Abstract
Three peptides, D1 (amino acid residues 175-201), D2 (a.a. 434-467), and TM (a.a. 1128-1159), corresponding to the spike protein (S) of severe acute respiratory syndrome corona virus (SARS CoV) were synthesized and their immunological functions were investigated in three different animals models (mice, guinea pigs, and rabbits). The peptides mixture formulated either with Freund's adjuvant or synthetic adjuvant Montanide ISA-51/oligodeoxy nucleotide CpG (ISA/CpG) could elicit antisera in immunized animals which were capable of inhibiting SARS/HIV pseudovirus entry into HepG2 cells. The neutralizing epitopes were identified using peptides to block the neutralizing effect of guinea pig antisera. The major neutralizing epitope was located on the D2 peptide, and the amino acid residue was fine mapped to 434-453. In BALB/c mice T-cell proliferation assay revealed that only D2 peptide contained T-cell epitope, the sequence of which corresponded to amino acid residue 434-448. The ISA/CpG formulation generated anti-D2 IgG titer comparable to those obtained from Freund's adjuvant formulation, but generated fewer antibodies against D1 or TM peptides. The highly immunogenic D2 peptide contains both neutralizing and Th cell epitopes. These results suggest that synthetic peptide D2 would be useful as a component of SARS vaccine candidates.
DOI: 10.1016/j.bbrc.2007.04.164
PubMed: 17506989
Affiliations:
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Chen</name></author><author><name sortKey="Chong, Pele" sort="Chong, Pele" uniqKey="Chong P" first="Pele" last="Chong">Pele Chong</name></author><author><name sortKey="Liu, Shih Jen" sort="Liu, Shih Jen" uniqKey="Liu S" first="Shih-Jen" last="Liu">Shih-Jen Liu</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Drug Design</term><term>Epitopes (chemistry)</term><term>Freund's Adjuvant</term><term>Guinea Pigs</term><term>Interferon-gamma (metabolism)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Peptides (chemistry)</term><term>Rabbits</term><term>Severe Acute Respiratory Syndrome (metabolism)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>T-Lymphocytes (metabolism)</term><term>Th1 Cells</term><term>Vaccines, Synthetic (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvant Freund</term><term>Animaux</term><term>Cochons d'Inde</term><term>Conception de médicament</term><term>Interféron gamma (métabolisme)</term><term>Lapins</term><term>Lymphocytes T (métabolisme)</term><term>Lymphocytes auxiliaires Th1</term><term>Peptides ()</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (métabolisme)</term><term>Tests de neutralisation</term><term>Vaccins synthétiques ()</term><term>Épitopes ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Epitopes</term><term>Peptides</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interféron gamma</term><term>Lymphocytes T</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Drug Design</term><term>Freund's Adjuvant</term><term>Guinea Pigs</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Rabbits</term><term>Th1 Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adjuvant Freund</term><term>Animaux</term><term>Cochons d'Inde</term><term>Conception de médicament</term><term>Lapins</term><term>Lymphocytes auxiliaires Th1</term><term>Peptides</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term><term>Vaccins synthétiques</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three peptides, D1 (amino acid residues 175-201), D2 (a.a. 434-467), and TM (a.a. 1128-1159), corresponding to the spike protein (S) of severe acute respiratory syndrome corona virus (SARS CoV) were synthesized and their immunological functions were investigated in three different animals models (mice, guinea pigs, and rabbits). The peptides mixture formulated either with Freund's adjuvant or synthetic adjuvant Montanide ISA-51/oligodeoxy nucleotide CpG (ISA/CpG) could elicit antisera in immunized animals which were capable of inhibiting SARS/HIV pseudovirus entry into HepG2 cells. The neutralizing epitopes were identified using peptides to block the neutralizing effect of guinea pig antisera. The major neutralizing epitope was located on the D2 peptide, and the amino acid residue was fine mapped to 434-453. In BALB/c mice T-cell proliferation assay revealed that only D2 peptide contained T-cell epitope, the sequence of which corresponded to amino acid residue 434-448. The ISA/CpG formulation generated anti-D2 IgG titer comparable to those obtained from Freund's adjuvant formulation, but generated fewer antibodies against D1 or TM peptides. The highly immunogenic D2 peptide contains both neutralizing and Th cell epitopes. These results suggest that synthetic peptide D2 would be useful as a component of SARS vaccine candidates.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chang, Yu Wen" sort="Chang, Yu Wen" uniqKey="Chang Y" first="Yu-Wen" last="Chang">Yu-Wen Chang</name><name sortKey="Chen, Hsin Wei" sort="Chen, Hsin Wei" uniqKey="Chen H" first="Hsin-Wei" last="Chen">Hsin-Wei Chen</name><name sortKey="Chen, Yi Ming A" sort="Chen, Yi Ming A" uniqKey="Chen Y" first="Yi-Ming A" last="Chen">Yi-Ming A. Chen</name><name sortKey="Chong, Pele" sort="Chong, Pele" uniqKey="Chong P" first="Pele" last="Chong">Pele Chong</name><name sortKey="Chou, Ai Hsiang" sort="Chou, Ai Hsiang" uniqKey="Chou A" first="Ai-Hsiang" last="Chou">Ai-Hsiang Chou</name><name sortKey="Leng, Chih Hsiang" sort="Leng, Chih Hsiang" uniqKey="Leng C" first="Chih-Hsiang" last="Leng">Chih-Hsiang Leng</name><name sortKey="Lin, Li Hsiu" sort="Lin, Li Hsiu" uniqKey="Lin L" first="Li-Hsiu" last="Lin">Li-Hsiu Lin</name><name sortKey="Lin, Min Han" sort="Lin, Min Han" uniqKey="Lin M" first="Min-Han" last="Lin">Min-Han Lin</name><name sortKey="Liu, Hsin Yu" sort="Liu, Hsin Yu" uniqKey="Liu H" first="Hsin-Yu" last="Liu">Hsin-Yu Liu</name><name sortKey="Liu, Shih Jen" sort="Liu, Shih Jen" uniqKey="Liu S" first="Shih-Jen" last="Liu">Shih-Jen Liu</name><name sortKey="Shih, Yi Ping" sort="Shih, Yi Ping" uniqKey="Shih Y" first="Yi-Ping" last="Shih">Yi-Ping Shih</name><name sortKey="Tsai, Jy Ping" sort="Tsai, Jy Ping" uniqKey="Tsai J" first="Jy-Ping" last="Tsai">Jy-Ping Tsai</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lien, Shu Pei" sort="Lien, Shu Pei" uniqKey="Lien S" first="Shu-Pei" last="Lien">Shu-Pei Lien</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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